Changelog
All notable changes to MyoToolkit will be documented on this page.
2026.03 — MyoPath
- Open-source release of MyoPath pipeline (GitHub)
- Four-tissue segmentation from H&E whole slide images: myofiber (Cellpose-SAM), fat (pixel classifier), nuclei (watershed), connective tissue (Boolean subtraction)
- 37 morphometric features across five biological categories
- Seven pathology indicators with NCI and Fiber CV as primary biomarkers
- MyoPath Score composite severity measure (AUC = 0.873 on external validation)
- Validated on 478 WSIs from two independent cohorts (GTEx n=399, HuashanMuscle n=79)
- QuPath + Python pipeline with batch processing and CLI support
2026.03 — MyoToolkit Website
- Initial release of the unified MyoToolkit documentation site
- Integrated MyoScore, MyoPath, MyoSpectrum, and ThighMRIseg under one platform
2024.04 — MyoSpectrum
- Published in Communications Biology, 2024, 7(1):438
- Integrative transcriptional atlas of 1,221 human skeletal muscles (9,231 genes)
- Continuous myopathy spectrum from healthy to severe muscle disease
- Novel featured genes: MGST1, AOX1, FASN, PRKCD, CHRNA1
- Clinical validation with CDM, LGMD R12, and FSHD cohorts
2023.06 — ThighMRIseg
- SegResNet-based 11-muscle thigh segmentation
- Multimodal support (IDEAL, T1, T2, STIR)
- Multiethnic training data (354 MRIs: Chinese, Finnish, German)
- MONAI Label deployment with OHIF and 3D Slicer integration